In the primary screening, up to 240 k molecules will be screened with a single readout at a typical screening concentration of 10 µM. Primary hits (typically ~ 500-1000) will be cherry-picked and tested again in triplicates at the same concentration to exclude technical artifacts. In addition, orthogonal assays (addressing the same target but using a different readout principle) and/or validation assays (e.g., addressing cellular toxicity or a control cell line) can be added for counter-screening. Confirmed hits (typically 100-200) will then be analyzed in a dose-dependent manner in the original assay as well as in all counter assays. Confirmed hits which reach a specified potency threshold (e.g., IC50 ≤ 1 µM) will be repurchased and tested again to verify a structure-activity relationship. If needed, confirmed hits can be further profiled in a range of secondary assays, i.e., using morphological profiling by means of the Cell Painting Assay (>> assay technology/cell painting), to gain a broader understanding of the compounds` cellular effects (and side-effects) and to further prioritize for low-throughput follow-up studies (e.g., transcriptomics/proteomics/mouse experiments).